貼壁細胞轉基因電極--PP35-2P 是設計用于原位貼壁細胞的電穿孔或在除樣品杯以外的其他容器內(nèi)進行較大量的電穿孔操作，使用電極在原位對貼壁細胞進行電穿孔避免了需使用化學品消化細胞以及低穿孔效率，中斷了細胞周期及細胞間的聯(lián)系等諸多問題．
貼壁細胞轉基因電極--PP35-2P 應用于哺乳動物細胞轉染，基因，蛋白或藥的傳遞，也可應用于植物和酵母。是一種可復用的電極，適用于配裝于6孔板的每個單孔或一個35mm直徑的培養(yǎng)皿，由13個間隔2mm的鍍金電極組成，適用于BTX各型電穿孔儀。
The Petri Pulser? PP35-2P is a reusable electroporationapplicator designed to fit into each single well of a 6-well plateor an individual 35mm diameter petri dish. The PP35-2P consists ofan electrode assembly embedded in a polyurethane holder with highvoltage electrode cables. The thin electrodes are gold plated anddesigned to maximize the surface area of electroporation. The highvoltage cables are connected with a BTX Pulse Generator, and theelectrode head inserted into the dish or well containing thesample. An electroporation pulse may then be delivered. The entireapplicator may be cleaned with mild detergent, and the electrodesmay be sterilized with ethanol and dried with acetone.
Applications
General Applications
The Petri Pulser PP35-2P is designed for the electroporation ofadherent cells in situ or as an alternative to cuvetteelectroporation for larger volumes or multiple cell samples. Theuse of the electrode for the electroporation of adherent cells insitu eliminates the need for trypsinization or mechanical removalof cells from their growth substrate. This eliminates associatedproblems including low plating efficiencies followingelectroporation, interruption of cell cycle and interruption ofintercellular communication.
Mammalian Cell Protein/Drug Electroincorporation
The Petri Pulser PP35-2P supplements our Model 366 Petri DishElectrode designed for 100 mm petri dishes, as the ideal electrodefor in situ electroincorporation of protein into mammaliancells.1,2,3 This electrode has been used for electroincorporationof peptides and proteins in CHO cells4 and vascular smooth musclecells5.
Mammalian Cell Transfections/Gene Therapy
The Petri Pulser PP35-2P is capable of supporting transfectionprotocols developed for our larger Model 366 Petri Dish Electrode,including protocols for BHK and HUVEC.6 The electrode itself hasbeen used for the transfection of 3T3-L1, Rat adipose cells, SF295human neuroglioblastoma and Cos 7 cells.7
Plant, Yeast and Bacterial Electroporation Applications
Applications involving plant, yeast and bacterial electroporationmay be carried out using the Petri Pulser. Scale up and multiplesample processing.
Technical Specifications
Standard Capabilities
Voltage Range: 0 - 300V
Pulse Length Range: 1 - 35 msec in PBS
Volume Range: 0.5 - 3.0 ml
Physical Characteristics
Weight: 6 oz
Number of Electrodes: 13
Electrode Thickness: 0.5 mm
Electrode Material: Gold Plated.
Generator Compatibility ECM 830, 630, 600, 399, 395, T 820
Ordering Information
System Model Number Available Configurations
PP35-2P Petri Pulser PP35-2P and Instruction Sheet
References
Marrero et al., Journal of Biological Chemistry, 270 (26):15734-15738 (1995)
Scheiffer et al., Journal of Biological Chemistry, 271 (17):10329-10333 (1996)
Marrero et al., Journal of Biological Chemistry, 272 (39):24684-24690 (1997)
Lobo, Errol, University of California, San Francisco, PersonalCommunication (1998)
Fukai, Masuko, Emory University, Personal Communication (1998)
BTX Protocols PR0165 and PR0330 (1998)
BTX Protocols PR0290, PR00286, PR0359, PR0360, PR0364 (1998)
